Leukopaks, Non-Mobilized

Access Fresh And Cryopreserved Leukopaks From Healthy And Diseased Donors For Research

Gain access to a growing network of providers for fresh and cryopreserved leukopaks from a large collective donor pool and diversity of phenotypes.

Select from a list of donor attributes such as blood type, HLA type, body mass index (BMI), gender, age, race, ethnicity, smoking (tobacco, vape or THC), or simply choose a standard Leukopak. You also have the ability to specify annotation requirements and exclusion criteria. Each Donor is assessed for medication, lifestyle, and medical history, tested for HBV, HCV, HIV and serological tests as a minimum, with an option to test for an expanded panel of infectious diseases.


The iSpecimen Advantage When Sourcing Leukopaks for Research

iSpecimen simplifies the process of sourcing reliable, highly-quality leukopaks while also offering many of the following benefits:

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    Specificity of Donor Attributes

    Refine inclusion and exclusion criteria from a wide range of attributes, including age, gender, race, ethnicity, blood type, smoker status, and more.

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    Diseased Mononuclear Cells

    iSpecimen also offers diseased mononuclear cells through its continually growing network of partners.

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    Optimized Cellularity & Cell Counts

    Available fresh or frozen, our leukopak collections typically yield over 10 billion WBCs within a sample volume of 300–500 ml.

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    Mobilized & Non-Mobilized Leukopaks

    Depending on your research and cell count requirements, choose from mobilized or non-mobilized leukopaks, all from your desired phenotype.

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    Cryopreserved, Healthy & Diagnosed Donors

    In addition to fresh leukopak collections, our network offers access to a large inventory of healthy and diseased cryopreserved, mobilized leukopaks.

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    Compliance Assurance

    Compliance is ensured. Providers are in conformance with U.S. CFR regulations under the oversight of an Independent Review Board (IRB).

Finding the Right Leukopak & Donor Match for Your Research

In the iSpecimen Marketplace®, researchers can request fresh or frozen mobilized and non-mobilized leukopaks from healthy and diagnosed patients fitting specific donor profiles, including but not limited to:

  • Blood Type
  • HLA Type
  • Body Mass Index (BMI)
  • Demographics (Gender, Age, Ethnicity, Race)
  • Smoking Status (tobacco, vape or THC).

Choosing the Best Leukopak Option for Your Research

The iSpecimen Marketplace provides access to fresh and frozen, healthy and diseased high-quality leukopaks, as defined by cell counts, percent cell viability, cellularity, validated packaging, and on-time delivery.

Collection lead times are typically two weeks for non-mobilized leukapheresis collections and six weeks for mobilized leukapheresis collections; lead times may vary depending on specificity of requested donor characteristics and collection criteria. Leukopaks can be shipped under cold or ambient conditions as requested, with an option to include a temperature logger.

Find a summary of options and additional details for each, below.

Fresh Non-Mobilized Leukopaks
  • Fresh collection, healthy donors
  • Full leukopaks, >300 ml
    See full details below
Fresh Mobilized Leukopaks
  • Fresh, healthy normal
  • Max yield, >50 billion cell count
  • Days 1-2-3-4-5 (second day collection available)
  • Full >300 ml
Cryopreserved Leukopaks, Healthy & Diagnosed
  • Healthy normal
  • Diagnosed, Cancer
  • Diagnosed, Autoimmune Diseases
  • See full details below

Fresh and Cryopreserved Leukopaks, Detailed Information

iSpecimen’s fresh and cryopreserved Leukopaks are provided for research and development use only, not for human therapeutic or diagnostic use.

Fresh Healthy Normal (Non-Mobilized) Leukopak Collection

Mobilized for Maximum Cell Yield

Mononuclear cells are collected and concentrated from peripheral blood of healthy consented donors according to IRB approved protocols using an automated leukapheresis system. During the procedure, an anticoagulant is added to venous blood to prevent blood from clotting as it passes through the apheresis device.

The enriched cell fraction consisting of pan T, NK, B cells, hematopoietic stem cells (CD34+), monocytes, dendritic, and other white blood cell types is collected and shipped in a sterile blood bag. Collection volumes for a full non-mobilized fresh Leukopak range from 300-500 ml and cell enumeration is typically >10 billion cells with cell yields as high as 28 billion, subject to donor variability.

Fresh Healthy Mobilized Leukopak Collection

Fresh Healthy Mobilized Leukopak Collection

To stimulate egress of hematopoietic stem cells (HSCs) from the bone marrow into peripheral blood, healthy consented donors are mobilized using granulocyte colony-stimulating factor (G-CSF, filgrastim/Neupogen) administered via subcutaneous injection on a daily basis. Depending on the specific mobilization protocol desired, healthy donors can be mobilized daily for one (1) to five (5) days followed by a single mononuclear cell collection plus an option for a second-day collection to maximize total cell yield from a single donor. For example, Day 1 Leukopak collection may typically yield >50 billion cells and Day 2 collections may typically yield >30 billion cells in a full Leukopak, subject to donor variability.

Cryopreserved Healthy Normal (Non-Mobilized) Leukopak Collection

Cryopreserved Healthy Normal (Non-Mobilized) Leukopak Collection

Freshly collected mononuclear cells are carefully cryopreserved using a 10% DMSO cryoprotectant in a sterile CRYO bag. Each Leukopak is cryopreserved at a US-FDA registered, AABB accredited facility using a controlled rate freezer according to a validated processing protocol. Each Leukopak is stored in the liquid nitrogen vapor phase at <-150 Deg. C to maintain optimal product quality. Cryopreserved Leukopaks are ready to be thawed and may allow for additional flexibility in conducting R&D studies depending on your changing schedule.

Cryopreserved Diagnosed Mobilized Leukopaks (Cancer)

Mobilized for Maximum Cell Yield Pre- and Post Thaw

iSpecimen offers a banked inventory of cryopreserved mobilized Leukopaks collected from early-stage cancer patients who have been mobilized using a 5-day GRANIX® (tbo-filgrastim) stimulation regimen. Leukopaks are available from patients diagnosed with ALL, CLL, MM, CML, Follicular Lymphoma, prostate and ovarian cancer, and renal failure. Each Leukopak is rapidly cryopreserved and stored in the liquid nitrogen vapor phase at <-150 Deg. C to maintain optimal quality. Each Leukopak varies in volume, total nucleated cell count, cellularity, and percent cell viability depending on the collection conditions used for each patient donor.

Cryopreserved Diagnosed Leukopaks (Autoimmune Disease)

Cryopreserved Diagnosed Leukopaks (Autoimmune Disease)

iSpecimen offers prospective cell collection capabilities for various types of autoimmune disease Leukopaks through our Marketplace network partners. Cryopreserved Leukopaks may be obtained from patients diagnosed with Crohn’s Disease, Psoriasis, Celiac Disease, Ulcerative Colitis, Lupus, Sjogren’s Syndrome, Diabetes, and others.

A New, Efficient Way of Sourcing Leukopaks

Managed ethics and compliance

We ensure that leukopak collection partners in our network conform to U.S. CFR regulations governing ethical procurement of human specimens, collection and processing of patient data, obtaining informed consent, full disclosure of risk, and appropriate donor compensation.

Access to target donors

In the iSpecimen Marketplace, conveniently select from a list of donor attributes such as blood type, HLA type, body mass index (BMI), gender, age, race, ethnicity, smoking (tobacco or vape or THC use) and infectious disease testing, or simply choose a standard Leukopak.

Dependable order management

In the iSpecimen Marketplace you can conveniently check order status with optional automated alerts, as orders move from collection into shipment and delivery. Of course, back-up donors are always scheduled to ensure you receive Leukopaks when you need them without delay.

Overnight Leukopak Shipping & Delivery

Recently, we arranged for the collection and overnight delivery of fresh whole leukopaks from adult non-smoker to a research organization on the east coast. If you need highly-specific, readily-available leukopaks, iSpecimen can help.

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The World is Your Biobank

Expand Your Patient Sets by Tapping into Our Global Network

Reach beyond restricted or limited patient populations to find the human tissue, blood products, and other biofluid specimens needed to accelerate your research.

Biospecimen Matching for Your Research

You need access to biospecimen samples that mirror your patient population’s genetics and physical attributes. With iSpecimen, you have more opportunities to source specific, diverse samples.


Access the Leukopaks You Need for Your Research