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Fresh and Cryopreserved Leukopacks

iSpecimen’s fresh and cryopreserved Leukopacks are provided for research and development use only. Not for human therapeutic or diagnostic use.

Fresh Healthy Normal (Non-Mobilized) Leukopack Collection Mononuclear cells are collected and concentrated from peripheral blood of healthy consented donors according to IRB approved protocols using an automated leukapheresis system. During the procedure, an anticoagulant is added to venous blood to prevent blood from clotting as it passes through the apheresis device. The enriched cell fraction consisting of pan T, NK, B cells, hematopoietic stem cells (CD34+), monocytes, dendritic, and other white blood cell types is collected and shipped in a sterile blood bag. Collection volumes for a full non-mobilized fresh Leukopack range from 300-500 ml and cell enumeration is typically >10 billion cells with cell yields as high as 28 billion, subject to donor variability.
Cryopreserved Healthy Normal (Non-Mobilized) Leukopack Collection Freshly collected mononuclear cells are carefully cryopreserved using a 10% DMSO cryoprotectant in a sterile CRYO bag. Each Leukopack is cryopreserved at a US-FDA registered, AABB accredited facility using a controlled rate freezer according to a validated processing protocol. Each Leukopack is stored in the liquid nitrogen vapor phase at <-150 Deg. C to maintain optimal product quality. Cryopreserved Leukopacks are ready to be thawed and may allow for additional flexibility in conducting R&D studies depending on your changing schedule.
Cryopreserved Diagnosed Mobilized Leukopacks (Cancer) iSpecimen offers a banked inventory of cryopreserved mobilized Leukopacks collected from early-stage cancer patients who have been mobilized using a 5-day GRANIX® (tbo-filgrastim) stimulation regimen. Peripheral blood mononuclear cells have been collected in a US-FDA registered AABB accredited stem cell collection and storage center using the Spectra Optia® apheresis system. Leukopacks are available from patients diagnosed with ALL, CLL, MM, CML, Follicular Lymphoma, prostate and ovarian cancer, and renal failure. Each Leukopack is rapidly cryopreserved using a 10% DMSO cryoprotectant in a sterile CRYO bag and a controlled rate freezer. Leukopacks are stored in the liquid nitrogen vapor phase at <-150 Deg. C to maintain optimal product quality. Redacted clinical reports are available for each diagnosed Leukopack.

Each Leukopack varies in volume, total nucleated cell (TNC) count, cellularity (e.g. CD34+ content), and percent cell viability depending on the collection conditions used for each patient donor.

Cryopreserved Diagnosed Leukopacks (Autoimmune Disease) iSpecimen offers prospective cell collection capabilities for various types of autoimmune disease Leukopacks through our Marketplace network partners. Cryopreserved Leukopacks may be obtained from patients diagnosed with Crohn’s Disease, Psoriasis, Celiac Disease, Ulcerative Colitis, Lupus, Sjogren’s Syndrome, Diabetes, and others.
Fresh Healthy Mobilized Leukopack Collection To stimulate egress of hematopoietic stem cells (HSCs) from the bone marrow into peripheral blood, healthy consented donors are mobilized using granulocyte colony-stimulating factor (G-CSF, filgrastim/Neupogen) administered via subcutaneous injection on a daily basis. Depending on the specific mobilization protocol desired, healthy donors can be mobilized daily for one (1) to five (5) days followed by a single mononuclear cell collection plus an option for a second-day collection to maximize total cell yield from a single donor. For example, Day 1 Leukopack collection may typically yield >50 billion cells and Day 2 collections may typically yield >30 billion cells in a full Leukopack, subject to donor variability.

More Specificity for Researchers Plus a Larger Total Healthy Donor Pool

Precision medicine research requires access to biomaterials that provide for greater specificity of certain donor attributes. By signing up under iSpecimen’s Marketplace, you gain access to a growing network of partners who operate multiple donor centers across different locations enabling access to a much larger collective donor pool and diversity of donor phenotypes. Avoid the time and expense associated with contacting multiple suppliers trying to source the specific donors and Leukopacks needed for your research.

Using our request a quote, conveniently select from a list of donor attributes such as blood type, HLA type, body mass index (BMI), gender, age, race, ethnicity, smoking (tobacco or vape or THC use) and infectious disease testing, or simply choose a standard Leukopack. You also have the ability to specify annotation requirements and exclusion criteria, as may be relevant to your research studies.

Easier Order Management and Dependability

Once you sign up under iSpecimen Marketplace you can login to conveniently check order status with optional automated alerts, as orders move from collection into shipment and delivery. Of course, back-up donors are always scheduled to ensure you receive Leukopacks when you need them without delay.


Each partner site within Marketplace is approved by iSpecimen according to strict qualification criteria to ensure conformance with U.S CFR regulations governing ethical procurement of human tissue, collection and processing of personal data, donor consent and safety, disclosure of risk, compensation, and use of tissue for life science research – all under the oversight of an Independent Review Board (IRB).

Diseased Mononuclear Cells

iSpecimen also offers diseased mononuclear cells through its continually growing network of partners.

Easily Select Healthy Donor Attributes for Prospective Collection

Once you’re logged into the iSpecimen Marketplace you’ll see how simple it is to select the Leukopack option you need – along with your desired healthy donor attributes – obtain pricing, and determine availability according to your desired delivery date. You can also quickly choose from a list of inclusion criteria and/or specify exclusion criteria for your application. For example, you may refine your search according to a donor’s age, gender, race, ethnicity, blood type, HLA type, BMI, and other profile information.C

Download our Data Sheet for details.