- Human Biospecimens
- For Researchers
- For Biospecimen Contributors
- For Patients
Normal (non-mobilized) Leukopack Collection
Mononuclear cells are collected and concentrated from peripheral blood of healthy consented donors according to IRB approved protocols using an automated leukapheresis system. During the procedure an anticoagulant is added to venous blood to prevent blood from clotting as it passes through the apheresis device. The enriched cell fraction consisting of pan T, NK, B cells, hematopoietic stem cells (CD34+), monocytes, dendritic and other white blood cell types is collected and shipped in a blood bag. Collection volumes for a full non-mobilized Leukopack range from 300-500 ml and cell enumeration is typically >10 billion cells with cell yields as high as 28 billion, subject to donor variability.
Mobilized Leukopack Collection
To stimulate egress of hematopoietic stem cells (HSCs) from the bone marrow into peripheral blood, healthy consented donors are mobilized using granulocyte colony stimulating factor (G-CSF, filgrastim/Neupogen) administered via subcutaneous injection on a daily basis. Depending on the specific mobilization protocol desired, healthy donors can be mobilized daily for one (1) to five (5) days followed by a single mononuclear cell collection plus an option for a second day collection to maximize total cell yield from a single donor. For example, Day 1 Leukopack collection may typically yield >50 billion cells and Day 2 collections may typically yield >30 billion cells in a full Leukopack, subject to donor variability.
Precision medicine research requires access to biomaterials that provide for greater specificity of certain donor attributes. Through the iSpecimen Marketplace, you gain access to a vast and growing network of iSpecimen partners who operate multiple donor centers across different locations, offering a large and diverse collective donor pool. Conveniently select from a list of donor attributes such as blood type, HLA type, body mass index (BMI), gender, age, race, ethnicity, smoker status (tobacco, vape or cannabis use) and infectious disease testing, or simply choose a standard Leukopack. You also have the ability to specify annotation requirements and exclusion criteria, as may be relevant to your research studies.
Avoid the time and expense associated with contacting multiple suppliers trying to source the specific donors and Leukopacks needed for your research. Our innovative iSpecimen Marketplace eliminates this often time-consuming, expensive, and onerous process by enabling you to manage all aspects of your specimen sourcing from a centralized location – from selection of donor criteria to quote requests, tracking shipments, accessing data, and even invoice management.
The iSpecimen Marketplace allows you to conveniently check order status – with optional automated alerts – as orders move from collection into shipment and delivery. Of course, back-up donors are always scheduled to ensure orders are received when you need them, without delay.
Each partner site within Marketplace is approved by iSpecimen according to strict qualification criteria to ensure conformance with U.S CFR regulations governing ethical procurement of human tissue, collection and processing of personal data, donor consent and safety, disclosure of risk, compensation, and use of tissue for life science research – all under the oversight of an Independent Review Board (IRB).
Once you’re logged into the iSpecimen Marketplace you’ll see how simple it is to select the Leukopack option you need – along with your desired healthy donor attributes – obtain pricing, and determine availability according to your desired delivery date. You can also quickly choose from a list of inclusion criteria and/or specify exclusion criteria for your application. For example, you may refine your search according to a donor’s age, gender, race, ethnicity, blood type, HLA type, BMI, and other profile information.C
Download our Data Sheet for details.